• If a protein has $\Delta G^{\circ}_{fold} = -28\ kJ\cdot mol^{-1}$, what fraction of the time will it be folded at $25\ ^{\circ}C$?

• You introduce a mutation that makes a new, favorable interaction that is only present in the folded state and that is worth $-11\ kJ \cdot mol^{-1}$ (on average). What is the fraction folded for this variant?
• Do you think this mutation would affect biological function? Why or why not?

$\Delta G = -RT\frac{P_{F}}{P_{U}}$

$\theta = \frac{P_{F}}{P_{F} +